alpha Adducin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38268

Novus Biologicals, part of Bio-Techne
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NBP2-38268
NBP2-38268-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all alpha Adducin Antibodies »

Product Summary for alpha Adducin Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PPSTPIKLEEDLVPEPTTGDDSDAATFKPTLPDLSPDEPSEALGFPMLEKEEEAHRP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alpha Adducin Antibody

Immunocytochemistry/ Immunofluorescence: alpha Adducin Antibody [NBP2-38268]

Immunocytochemistry/ Immunofluorescence: alpha Adducin Antibody [NBP2-38268]

Immunocytochemistry/Immunofluorescence: alpha Adducin Antibody [NBP2-38268] - Staining of human cell line U-2 OS shows localization to nuclear bodies & plasma membrane.
Immunohistochemistry-Paraffin: alpha Adducin Antibody [NBP2-38268]

Immunohistochemistry-Paraffin: alpha Adducin Antibody [NBP2-38268]

Immunohistochemistry-Paraffin: alpha Adducin Antibody [NBP2-38268] - Staining of human colon.
Immunohistochemistry: alpha Adducin Antibody [NBP2-38268]

Immunohistochemistry: alpha Adducin Antibody [NBP2-38268]

Immunohistochemistry: alpha Adducin Antibody [NBP2-38268] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
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Applications for alpha Adducin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha Adducin

Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; alpha and beta, or alpha and gamma subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).

Alternate Names

ADDA, adducin 1 (alpha), alpha-adducin, erythrocyte adducin alpha subunit, Erythrocyte adducin subunit alpha, MGC3339, MGC44427

Gene Symbol

ADD1

UniProt

P35611 (Mouse)

Additional alpha Adducin Products

Product Documents for alpha Adducin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha Adducin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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