alpha-Aminoadipate Aminotransferase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-99587

Novus Biologicals, part of Bio-Techne
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NB110-99587
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all alpha-Aminoadipate Aminotransferase Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KPEDAKNPQKNTPK corresponding to internal region according to NP_057312.1, NP_872603.1.

Specificity

Both reported variants represent identical protein (NP_057312.1and NP_872603.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for alpha-Aminoadipate Aminotransferase Antibody

Western Blot: alpha-Aminoadipate Aminotransferase Antibody [NB110-99587]

Western Blot: alpha-Aminoadipate Aminotransferase Antibody [NB110-99587]

Western Blot: alpha-Aminoadipate Aminotransferase Antibody [NB110-99587] - (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for alpha-Aminoadipate Aminotransferase Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in human liver lysates (calculated MW of 47.4 kDa band according to NP_057312.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha-Aminoadipate Aminotransferase

Aminoadipate aminotransferase (AADAT) is a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist.

Long Name

alpha-Aminoadipate Aminotransferase

Alternate Names

AADAT, alphaAminoadipate Aminotransferase, KAT2

Gene Symbol

AADAT

UniProt

NP_057312.1 (Human)

Additional alpha-Aminoadipate Aminotransferase Products

Product Documents for alpha-Aminoadipate Aminotransferase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha-Aminoadipate Aminotransferase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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