alpha-Aminoadipate Aminotransferase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89627

Novus Biologicals, part of Bio-Techne
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NBP1-89627
NBP1-89627-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all alpha-Aminoadipate Aminotransferase Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSWLKQLQIKLHNPPTIHYPPSQGQMDLCVTSGSQQGLCKVFEMIINPGDNVLLDEPAYSGTLQSLHPLGCNIINVASDESGIVPD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alpha-Aminoadipate Aminotransferase Antibody

Immunohistochemistry-Paraffin: alpha-Aminoadipate Aminotransferase Antibody [NBP1-89627]

Immunohistochemistry-Paraffin: alpha-Aminoadipate Aminotransferase Antibody [NBP1-89627]

Immunohistochemistry-Paraffin: alpha-Aminoadipate Aminotransferase Antibody [NBP1-89627] - Staining in human liver and pancreas tissues using anti-AADAT antibody. Corresponding AADAT RNA-seq data are presented for the same tissues.
Western Blot: alpha-Aminoadipate Aminotransferase Antibody [NBP1-89627]

Western Blot: alpha-Aminoadipate Aminotransferase Antibody [NBP1-89627]

Western Blot: alpha-Aminoadipate Aminotransferase Antibody [NBP1-89627] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-8
Immunocytochemistry/ Immunofluorescence: alpha-Aminoadipate Aminotransferase Antibody [NBP1-89627]

Immunocytochemistry/ Immunofluorescence: alpha-Aminoadipate Aminotransferase Antibody [NBP1-89627]

Immunocytochemistry/Immunofluorescence: alpha-Aminoadipate Aminotransferase Antibody [NBP1-89627] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
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Applications for alpha-Aminoadipate Aminotransferase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Aminoadipate Aminotransferase

Aminoadipate aminotransferase (AADAT) is a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist.

Long Name

alpha-Aminoadipate Aminotransferase

Alternate Names

AADAT, alphaAminoadipate Aminotransferase, KAT2

Gene Symbol

AADAT

Additional alpha-Aminoadipate Aminotransferase Products

Product Documents for alpha-Aminoadipate Aminotransferase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha-Aminoadipate Aminotransferase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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