alpha COP I Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-2913

Novus Biologicals, part of Bio-Techne
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NB120-2913
NB120-2913-0.025mg
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all alpha COP I Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues M(1) L T K F E T K S A R V K G L S F H P(19) C of rat alpha-COP.

Localization

Cytoplasmic and Golgi Apparatus

Specificity

Detects coatomer-protein I alpha (COP I alpha).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

138 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha COP I Antibody

Immunocytochemistry/ Immunofluorescence: alpha COP I Antibody [NB120-2913]

Immunocytochemistry/ Immunofluorescence: alpha COP I Antibody [NB120-2913]

Immunocytochemistry/Immunofluorescence: alpha COP I Antibody [NB120-2913] - HeLa cells.

Applications for alpha COP I Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

3 ug/ml

Western Blot

1 ug/ml
Application Notes
WB: Detects an approx. 140 kDa protein representing COPI alpha from PC-12 cell extract. Also detects an unidentified protein at approx. 100 kDa. ICC: Staining of alpha COP I results in alpha COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha COP I

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.

Alternate Names

alpha coat protein, Alpha-coat protein, Alpha-COP, coatomer protein complex, subunit alpha, coatomer subunit alpha, FLJ26320, HEPCOP, HEP-COPalpha-COP, xenin

Entrez Gene IDs

1314 (Human); 304978 (Rat)

Gene Symbol

COPA

UniProt

G3V6T1 (Rat)

Additional alpha COP I Products

Product Documents for alpha COP I Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha COP I Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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