Alpha Dystroglycan Antibody (2238)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49634

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Rabbit

Cited:

Human, Mouse

Applications

Validated:

Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2238

Concentration

0.1 mg/ml

View all Alpha Dystroglycan Antibodies »

Product Specifications

Immunogen

Monoclonal antibody 2238 recognizes a glycoepitope unique to brain alpha-dystroglycan.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

The monoclonal antibody 2238 is specific for a glycoepitope on brain bovine alpha-dystroglycan, which is absent on alpha-dystroglycan expressed in all other tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Alpha Dystroglycan Antibody (2238)

Immunohistochemistry: Alpha Dystroglycan Antibody (2238) [NBP1-49634]

Immunohistochemistry: Alpha Dystroglycan Antibody (2238) [NBP1-49634]

Immunohistochemistry: Alpha Dystroglycan Antibody (2238) [NBP1-49634] - Endothelial B-catenin GOF does not affect astrocytic endfoot polarization of a-dystroglycan (a-Dag) and Kir4.1 within the subfornical organ (SFO). Striatal BBB-vessel showing a polarized distribution of a-Dag and Kir4.1 in AC endfeet. Lumen is stained by Podxl (asterisk) (A). Coronal overview of the subfornical organ (SFO) (B); rectangular inset demarcates area for higher magnification in (C). Dashed lines outline SFO vessels. Scale bar show 2 um (A), 50 um (B), 10 um (C). Image collected and cropped by CiteAb from the following publication (elifesciences.org/articles/43818), licensed under a CC-BY license.
Alpha Dystroglycan Antibody (2238)

Immunohistochemistry: Alpha Dystroglycan Antibody (2238) [NBP1-49634] -

Endothelial beta-catenin GOF does not affect astrocytic endfoot polarization of alpha-dystroglycan ( alpha-Dag) and Kir4.1 within the subfornical organ (SFO).Striatal BBB-vessel showing a polarized distribution of alpha-Dag and Kir4.1 in AC endfeet. Lumen is stained by Podxl (asterisk) (A). Coronal overview of the subfornical organ (SFO) (B); rectangular inset demarcates area for higher magnification in (C). Dashed lines outline SFO vessels. Scale bar show 2 µm (A), 50 µm (B), 10 µm (C).
Alpha Dystroglycan Antibody (2238)

Immunocytochemistry/ Immunofluorescence: Alpha Dystroglycan Antibody (2238) [NBP1-49634] -

Immunocytochemistry/ Immunofluorescence: Alpha Dystroglycan Antibody (2238) [NBP1-49634] - Endothelial beta-catenin GOF does not affect astrocytic endfoot polarization of alpha-dystroglycan ( alpha-Dag) & Kir4.1 within the subfornical organ (SFO).Striatal BBB-vessel showing a polarized distribution of alpha-Dag & Kir4.1 in AC endfeet. Lumen is stained by Podxl (asterisk) (A). Coronal overview of the subfornical organ (SFO) (B); rectangular inset demarcates area for higher magnification in (C). Dashed lines outline SFO vessels. Scale bar show 2 µm (A), 50 µm (B), 10 µm (C). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30932814), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Alpha Dystroglycan Antibody (2238)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This antibody works in Western Blot, Immunoassay and Immunohistochemistry-Paraffin WB:it recognizes alpha-DG as protein of ~130 kD, especially after enrichment of the lysates for dystroglycans Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27015747)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.2 um filtered solution in PBS and 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Alpha Dystroglycan

Monoclonal antibody 2238 recognizes a glycoepitope unique to brain alpha-dystroglycan. Alpha-dystroglycan (alpha-DG), also known as dystrophin-associated glycoprotein, is a laminin-binding protein of ~156 kDa (including glyco-groups). Alpha-DG is a component of the dystroglycan complex, which is involved in early development, morphogenesis and in the pathogenesis of muscular dystrophies. Alpha- and beta-DG are encoded by a single gene and are derived from a precursor polypeptide by posttranslational cleavage. Beta-DG is an integral membrane protein, whereas alpha-DG is membrane-associated through its noncovalent interaction with the extracellular domain of beta-DG. The alpha- and beta-DGs provide important physical linkages between components of basement membranes and cytoplasmic proteins that bind to the actin cytoskeleton. Alpha-DG is a heavily glycosylated, mucin-like protein anchored on the extracellular surface of the myotube, where it may provide linkage between the sarcolemma and extracellular matrix (ECM). Alpha-DG is expressed in a variety of fetal and adult tissues. Tissue-specific glycosylation modifies the laminin specificity of alpha-DG. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alpha-DG has been shown to colocalize with laminin in skeletal and cardiac muscle and a number of other cells including peripheral nerve, astrocytes, Purkinje neurons and kidney epithelium. Laminin-10/11 was shown to bind preferentially to brain alpha-DG. In Duchenne muscular dystrophy, the expression of alpha-DG is dramatically reduced leading to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. In the central nervous system, dystroglycan functions as a dual receptor for agrin and laminin-2 for instance in the Schwann cell membrane. Furthermore, defects in dystroglycan are central to the pathogenesis of structural and functional brain abnormalities seen in congenital muscular dystrophies (CMD).

Alternate Names

A3a, AGRNR, DAG156DAG, dystroglycan, dystroglycan 1 (dystrophin-associated glycoprotein 1), Dystrophin-associated glycoprotein 1

Entrez Gene IDs

1605 (Human); 13138 (Mouse); 114489 (Rat); 281439 (Bovine); 100009278 (Rabbit)

Gene Symbol

DAG1

UniProt

O18738 (Bovine)

Additional Alpha Dystroglycan Products

Product Documents for Alpha Dystroglycan Antibody (2238)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Alpha Dystroglycan Antibody (2238)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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