alpha-Internexin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-93355

Novus Biologicals, part of Bio-Techne
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NB110-93355
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all alpha-Internexin Antibodies »

Product Specifications

Immunogen

Recombinant rat alpha-internexin expressed in and purified from E. coli. Accession # P23565

Reactivity Notes

Reactivity assumed based on 100% sequence homology to a wide variety of mammalian species.

Specificity

Specific for endogenous levels of the ~66 kDa alpha-Internexin protein. Minor bands at ~150 kDa are probably covalent dimers and bands at ~50 kDa represent alpha-internexin breakdown products.

Marker

Immature Neuronal Marker

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for alpha-Internexin Antibody

Western Blot: alpha-Internexin Antibody [NB110-93355]

Western Blot: alpha-Internexin Antibody [NB110-93355]

Western Blot: alpha-Internexin Antibody [NB110-93355] - Western blot of rat cortex lysate showing specific immunolabeling of the ~ 66k alpha internexin protein.
Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody [NB110-93355]

Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody [NB110-93355]

Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody [NB110-93355] - Immunofluorescence of a section of rat cerebellum showing labeling of axons of granule cell and dendrites of Purkinje with alpha-internexin, labeling of the myelin sheaths around the axons with MBP, and additional nuclear staining with DAPI (blue).
Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody [NB110-93355]

Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody [NB110-93355]

Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody [NB110-93355] - Immunostaining of cultured neurons and glia showing specific labeling of neuronal processes (red) using our alpha-internexin antibody and microglia (green) with a coronin 1a antibody.

Applications for alpha-Internexin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:5000

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

PBS

Preservative

0.07% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha-Internexin

Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).

Alternate Names

alphaInternexin, INA, NEF5, NF-66, TXBP-1

Entrez Gene IDs

9118 (Human); 226180 (Mouse); 24503 (Rat)

Gene Symbol

INA

UniProt

P23565 (Rat)

Additional alpha-Internexin Products

Product Documents for alpha-Internexin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha-Internexin Antibody

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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