alpha-Internexin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81007

Novus Biologicals, part of Bio-Techne
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NBP1-81007
NBP1-81007-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all alpha-Internexin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ALDIEIAAYRKLLEGEETRFSTSGLSISGLNPLPNPSYLLPPRILSATTSKVSSTGLSLKKEEEEEEASKVASKKTSQIGESFEEILEETVISTKKTEKSNIEETTISSQ

Reactivity Notes

Reactivity reported in scientific literature (PMID: 19351664)

Marker

Immature Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alpha-Internexin Antibody

Western Blot: alpha-Internexin Antibody [NBP1-81007]

Western Blot: alpha-Internexin Antibody [NBP1-81007]

Western Blot: alpha-Internexin Antibody [NBP1-81007] - Analysis in human cell line SH-SY5Y.
Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody [NBP1-81007]

Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody [NBP1-81007]

Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody [NBP1-81007] - Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm, nuclear membrane & intermediate filaments.
Immunohistochemistry-Paraffin: alpha-Internexin Antibody [NBP1-81007]

Immunohistochemistry-Paraffin: alpha-Internexin Antibody [NBP1-81007]

Immunohistochemistry-Paraffin: alpha-Internexin Antibody [NBP1-81007] - Staining in human cerebral cortex and kidney tissues . Corresponding INA RNA-seq data are presented for the same tissues.
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Applications for alpha-Internexin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 21246521). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Internexin

Alpha-internexin is a Class IV intermediate filament, related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development procedes. However, many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H, and some mature neurons only express the alpha-internexin subunit of neurofilament.

The very early developmental expression of alpha-internexin suggests that its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. Therefore, alpha-internexin antibodies serve as unique probes to study and classify neuronal types and follow their processes in tissue sections and culture.

Alternate Names

alphaInternexin, INA, NEF5, NF-66, TXBP-1

Entrez Gene IDs

9118 (Human)

Gene Symbol

INA

UniProt

Q16352 (Human)

Additional alpha-Internexin Products

Product Documents for alpha-Internexin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha-Internexin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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