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alpha-N-acetylglucosaminidase/NAGLU Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19465

Novus Biologicals, part of Bio-Techne
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NBP2-19465

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for alpha-N-acetylglucosaminidase/NAGLU Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha-N-acetylglucosaminidase/NAGLU. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-N-acetylglucosaminidase/NAGLU Antibody

Western Blot: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465]

Western Blot: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465]

Western Blot: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465]

Immunocytochemistry/ Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465]

Immunocytochemistry/Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody [N3C1], Internal diluted at 1:200. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (NBP2-43559) diluted at 1:200. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465]

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465]

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465] - Mouse brain. NAGLU antibody [N3C1], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for alpha-N-acetylglucosaminidase/NAGLU Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha-N-acetylglucosaminidase/NAGLU

This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq]

Alternate Names

alphaNacetylglucosaminidase, NAG, NAGLU, UFHSD1

Gene Symbol

NAGLU

UniProt

Additional alpha-N-acetylglucosaminidase/NAGLU Products

Product Documents for alpha-N-acetylglucosaminidase/NAGLU Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for alpha-N-acetylglucosaminidase/NAGLU Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.