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alpha-Sarcoglycan Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92438

Novus Biologicals, part of Bio-Techne
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NBP2-92438-0.02ml
NBP2-92438-0.1ml

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 60-290 of human alpha-Sarcoglycan (NP_000014.1). ITYHAHLQGHPDLPRWLRYTQRSPHHPGFLYGSATPEDRGLQVIEVTAYNRDSFDTTRQRLVLEIGDPEGPLLPYQAEFLVRSHDAEEVLPSTPASRFLSALGGLWEPGELQLLNVTSALDRGGRVPLPIEGRKEGVYIKVGSASPFSTCLKMVASPDSHARCAQGQPPLLSCYDTLAPHFRVDWCNVTLVDKSVPEPADEVPTPGDGILEHDPFFCPPTEAPDRDFLVDA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for alpha-Sarcoglycan Antibody - Azide and BSA Free

Western Blot: alpha-Sarcoglycan AntibodyAzide and BSA Free [NBP2-92438]

Western Blot: alpha-Sarcoglycan AntibodyAzide and BSA Free [NBP2-92438]

Western Blot: alpha-Sarcoglycan Antibody [NBP2-92438] - Analysis of extracts of various cell lines, using alpha-Sarcoglycan at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: alpha-Sarcoglycan Antibody - Azide and BSA Free [NBP2-92438]

Immunocytochemistry/ Immunofluorescence: alpha-Sarcoglycan Antibody - Azide and BSA Free [NBP2-92438]

Immunocytochemistry/Immunofluorescence: alpha-Sarcoglycan Antibody [NBP2-92438] - Analysis of C6 cells using alpha Sarcoglycan (alpha Sarcoglycan (SGCA)) Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: alpha-Sarcoglycan Antibody - Azide and BSA Free [NBP2-92438]

Immunocytochemistry/ Immunofluorescence: alpha-Sarcoglycan Antibody - Azide and BSA Free [NBP2-92438]

Immunocytochemistry/Immunofluorescence: alpha-Sarcoglycan Antibody [NBP2-92438] - Analysis of U2OS cells using alpha Sarcoglycan (alpha Sarcoglycan (SGCA)) Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for alpha-Sarcoglycan Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha-Sarcoglycan

The dystrophin-glycoprotein complex (DGC) comprises a group of proteins that are critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Components of the DGC include dystrophin (MIM 300377), which is deficient in Duchenne muscular dystrophy (DMD; MIM 310200); syntrophins (e.g., MIM 600026); dystroglycans (MIM 128239); and sarcoglycans, such as adhalin, a 50-kD transmembrane protein (Roberds et al., 1993 [PubMed 8226900]).[supplied by OMIM]

Alternate Names

50-DAG, Adhalin, ADL, alphaSarcoglycan, DAG2, DMDA2, Dystroglycan-2, LGMD2D, SCARMD1, SGCA

Gene Symbol

SGCA

Additional alpha-Sarcoglycan Products

Product Documents for alpha-Sarcoglycan Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for alpha-Sarcoglycan Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov