alpha Tubulin Antibody (YL1/2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80571

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Avian, C. elegans, Drosophila, Invertebrate, Mammal, Monkey, Primate, Yeast

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Radioimmunoassay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # YL1/2

Format

Azide and BSA Free

Concentration

1 mg/ml

View all alpha Tubulin Antibodies »

Product Specifications

Immunogen

This alpha Tubulin Antibody (YL1/2) was developed against full length native protein (purified) (S. cerevisiae).

Epitope

A linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).

Reactivity Notes

S. cerevisiae, S. pombe, Slime molds, Allium. Other species have not been tested. Expected to react with most eukaryotes due to sequence identity. Drosophila reactivity reported in scientific literature (PMID: 24019759). C. elegans reactivity reported in scientific literature (PMID: 29118344). Toxoplasma gondii reactivity reported by customer review.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha Tubulin Antibody (YL1/2) - Azide and BSA Free

Western Blot: alpha Tubulin Antibody (YL1/2)Azide and BSA Free [NBP2-80571]

Western Blot: alpha Tubulin Antibody (YL1/2)Azide and BSA Free [NBP2-80571]

Western Blot: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Western Blot analysis of whole cell lysates of HeLa, NIH-3T3 and PC-12 cell lines using Tubulin antibody (clone YL1/2). Alpha tubulin molecular weight: 50 kDa. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Tubulin YL1/2 antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue). Image from the standard format of this antibody.
Immunohistochemistry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunohistochemistry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunohistochemistry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Tubulin antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain. Image from the standard format of this antibody.
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Applications for alpha Tubulin Antibody (YL1/2) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:1000

Flow Cytometry

2-5 ug/0.1x10^6 cells

Immunocytochemistry/ Immunofluorescence

1:1000-1:10000

Immunohistochemistry

1:200

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10-1:500

Western Blot

1:5000-1:10000
Application Notes
This Tubulin Antibody (YL1/2) is useful for Western blot, Immunoprecipitation, Immunohistochemistry on frozen and paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Radioimmunoassay and ELISA. NB600-506 is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in Immunocytochemistry.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha Tubulin

Tubulin is the major building block of microtubules. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. The tubulin superfamily is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa.

Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).

References

1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921

2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718

Long Name

Tubulin Alpha 1a

Alternate Names

Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1

Gene Symbol

TUBA1A

Additional alpha Tubulin Products

Product Documents for alpha Tubulin Antibody (YL1/2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha Tubulin Antibody (YL1/2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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