AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70454

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6A9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all AlphaB Crystallin/CRYAB Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free

Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9)Azide and BSA Free [NBP2-70454]

Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9)Azide and BSA Free [NBP2-70454]

Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Western blot analysis of extracts (35ug) from 9 different cell lines by using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454]

Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454]

Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunofluorescent staining of HT29 cells using AlphaB Crystallin/CRYAB Antibody (OTI6A9) (NBP2-70454).
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free [NBP2-70454] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using AlphaB Crystallin/CRYAB Antibody (OTI6A9). (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, 0454)
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Applications for AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AlphaB Crystallin/CRYAB

Crystallin AB is a member of the small heat shock protein family and expressed in several tissues including brain, kidney cardiac and skeletal muscle. Crystallin AB functions as a molecular chaperone and in intracellular architecture. Crystallin AB has shown to have interactions with CRYAA, CRYGC, CRYBB2, CRYBA1 and HSPB8. Defects in crystalline AB have been linked to cause myopathy myofibrillar type 2, cataract posterior polar type 2, and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Alternate Names

CRYA2, CRYAB, CTPP2, HSPB5

Gene Symbol

CRYAB

Additional AlphaB Crystallin/CRYAB Products

Product Documents for AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AlphaB Crystallin/CRYAB Antibody (OTI6A9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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