AlphaB Crystallin/CRYAB Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2519
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Chicken (100%), Guinea Pig (100%), Porcine (100%), Primate (100%), Rabbit (100%), Rat (100%), Sheep (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.08 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an internal region of the human Crystallin AB protein (within residues 70-140). [Swiss-Prot P02511]
Reactivity Notes
Predicted to react with rat, sheep, rabbit, monkey, guinea pig, chicken, and pig based on 100% sequence homology. Immunogen sequence has 81% homology with Zebrafish.
Localization
Cytoplasmic
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AlphaB Crystallin/CRYAB Antibody
Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519]
Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (https://doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519]
Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519]
Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Detection of CryAB using NB 100-2519 (0.5ug/ml). Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.Applications for AlphaB Crystallin/CRYAB Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:150. Use reported in scientific literature
Western Blot
0.5-1.0 ug/ml
Application Notes
In Western blot analysis, a band is seen at ~20 kDa. There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex. **CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1.08 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: AlphaB Crystallin/CRYAB
Alternate Names
CRYA2, CRYAB, CTPP2, HSPB5
Gene Symbol
CRYAB
Additional AlphaB Crystallin/CRYAB Products
Product Documents for AlphaB Crystallin/CRYAB Antibody
Product Specific Notices for AlphaB Crystallin/CRYAB Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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