AlphaB Crystallin/CRYAB [p Ser45] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB120-5598

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Validated:

Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

LYOPH mg/ml

View all AlphaB Crystallin/CRYAB Antibodies »

Product Specifications

Immunogen

Synthetic phopshopeptide corresponding to residues L(44) S(p) P F Y L R P P S F(54) C of human alpha-B Crystallin.

Modification

p Ser45

Localization

Cytoplasmic

Specificity

This does not detect the unphosphorylated form of the protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AlphaB Crystallin/CRYAB [p Ser45] Antibody - BSA Free

Western Blot: AlphaB Crystallin/CRYAB [p Ser45] AntibodyBSA Free [NB120-5598]

Western Blot: AlphaB Crystallin/CRYAB [p Ser45] AntibodyBSA Free [NB120-5598]

Western Blot: AlphaB Crystallin/CRYAB [p Ser45] Antibody [NB120-5598] - Analysis of recombinant bovine phospho-alpha-B crystallin (Ser45). Lane 1 is phospho-alpha-B crystallin (Ser45) and lane 2 is unphosphorylated alpha-B crystallin.

Applications for AlphaB Crystallin/CRYAB [p Ser45] Antibody - BSA Free

Application
Recommended Usage

Immunofluorescence

5 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

8 ug/ml

Western Blot

0.5 ug/ml
Application Notes
WB: Detects an approx. 20 kDa representing recombinant bovine phospho-alpha-B crystallin (Ser45). IF: Staining of phospho-alpha-B crystallin (Ser45) in U373 MG cells results in cytoplasmic staining.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute to desired concentration.

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AlphaB Crystallin/CRYAB

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander’s disease where it accumulates in brain cells of those afflicted.

Alternate Names

CRYA2, CRYAB, CTPP2, HSPB5

Entrez Gene IDs

1410 (Human); 12955 (Mouse); 25420 (Rat)

Gene Symbol

CRYAB

Additional AlphaB Crystallin/CRYAB Products

Product Documents for AlphaB Crystallin/CRYAB [p Ser45] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AlphaB Crystallin/CRYAB [p Ser45] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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