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ALPPL2 Antibody (2B3)

Novus Biologicals, part of Bio-Techne | Catalog # H00000251-M07

Novus Biologicals, part of Bio-Techne
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H00000251-M07

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Sandwich ELISA, Sandwich ELISA Capture, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2B3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ALPPL2 (NP_112603, 365 a.a. ~ 454 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGETHAGE

Specificity

ALPPL2 - alkaline phosphatase, placental-like 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ALPPL2 Antibody (2B3)

Western Blot: ALPPL2 Antibody (2B3) [H00000251-M07]

Western Blot: ALPPL2 Antibody (2B3) [H00000251-M07]

Western Blot: ALPPL2 Antibody (2B3) [H00000251-M07] - Analysis of ALPPL2 expression in transfected 293T cell line by ALPPL2 monoclonal antibody (M07), clone 2B3. Lane 1: ALPPL2 transfected lysatE (57.5 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: ALPPL2 Antibody (2B3) [H00000251-M07]

Immunohistochemistry-Paraffin: ALPPL2 Antibody (2B3) [H00000251-M07]

Immunohistochemistry-Paraffin: ALPPL2 Antibody (2B3) [H00000251-M07] - Analysis of monoclonal antibody to ALPPL2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
Western Blot: ALPPL2 Antibody (2B3) [H00000251-M07]

Western Blot: ALPPL2 Antibody (2B3) [H00000251-M07]

Western Blot: ALPPL2 Antibody (2B3) [H00000251-M07] - ALPPL2 over-expressed 293 cell line. Lane 2 cotransfected with ALPPL2 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000251-M07. GAPDH (36.1 kDa) used as loading control.

Applications for ALPPL2 Antibody (2B3)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ALPPL2

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase.

Alternate Names

Alkaline phosphatase Nagao isozyme, alkaline phosphatase, placental-like, alkaline phosphatase, placental-like 2, ALP-1, ALPG, ALPPL, EC 3.1.3.1, GCAP, Germ cell alkaline phosphatase, Nagao isozyme, Placental alkaline phosphatase-like, placental-like alkaline phosphatase, PLAP-like, testicular and thymus alkaline phosphatase

Entrez Gene IDs

251 (Human)

Gene Symbol

ALPG

OMIM

171810 (Human)

Additional ALPPL2 Products

Product Documents for ALPPL2 Antibody (2B3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ALPPL2 Antibody (2B3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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