Aly Antibody (2G8)

Novus Biologicals, part of Bio-Techne | Catalog # H00010189-M03

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
H00010189-M03
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2G8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Aly Antibodies »

Product Specifications

Immunogen

ALYREF (NP_005773.2, 106 a.a. ~ 193 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GKLLVSNLDFGVSDADIQELFAEFGTLKKAAVHYDRSGRSLGTADVHFERKADALKAMKQYNGVPLDGRPMNIQLVTSQIDAQRRPAQ

Specificity

This product is specific for Human THOC4 monoclonal antibody (M03), clone 2G8 [Gene ID: 10189].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Aly Antibody (2G8)

Western Blot: Aly Antibody (2G8) [H00010189-M03]

Western Blot: Aly Antibody (2G8) [H00010189-M03]

Western Blot: Aly Antibody (2G8) [H00010189-M03] - Analysis of ALYREF expression in Jurkat.
Immunocytochemistry/ Immunofluorescence: Aly Antibody (2G8) [H00010189-M03]

Immunocytochemistry/ Immunofluorescence: Aly Antibody (2G8) [H00010189-M03]

Immunocytochemistry/Immunofluorescence: Aly Antibody (2G8) [H00010189-M03] - Analysis of monoclonal antibody to ALYREF on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Aly Antibody (2G8) [H00010189-M03] - Detection limit for recombinant GST tagged ALYREF is 0.1 ng/ml as a capture antibody.

Applications for Aly Antibody (2G8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Mouse monoclonal antibody raised against a partial recombinant THOC4.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Aly

Pre-mRNA splicing plays an important role in regulation of gene expression. During the splicing process, specific proteins are recruited to the mRNA and assembled into a complex of mRNA-protein near the exon-exon junction. This complex is termed the mRNA-protein complex (mRNP) or the exon-exon junction complex. The proteins that are found in this complex include: Y14, magoh, Aly/REF, RNPS1, Upf3, and DEK.1-5 Aly (also known as mREF1-I) is the mammalian homologue of the yeast mRNA export factor Yra1p.6-7 It is a 233 amino acid RNAbinding protein that contains a RNA-binding domain and is recruited to the mRNP complexes during the splicing. Excess of recombinant expression of Aly in the cells increases both the rate and efficiency of spliced and unspliced mRNA export (in vivo) from the nucleus to the cytoplasm. In contrast to the Y14 protein, Aly is dissociated from the mRNAs after the export to the cytoplasm.1, 5-6

Alternate Names

Ally of AML-1 and LEF-1, ALY/REF, ALYBEFbZIP enhancing factor, bZIP-enhancing factor BEF, REF, THO complex 4, THO complex subunit 4, tho4, Transcriptional coactivator Aly/REF

Gene Symbol

ALYREF

UniProt

NP_005773.2 (Human)

Additional Aly Products

Product Documents for Aly Antibody (2G8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Aly Antibody (2G8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.