AMBRA1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92849

Novus Biologicals, part of Bio-Techne
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NBP2-92849-0.02ml
NBP2-92849-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AMBRA1 Antibodies »

Product Summary for AMBRA1 Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human Ambra1 (NP_060219.2). GAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMBRA1 Antibody - BSA Free

Western Blot: AMBRA1 AntibodyBSA Free [NBP2-92849]

Western Blot: AMBRA1 AntibodyBSA Free [NBP2-92849]

Western Blot: AMBRA1 Antibody [NBP2-92849] - Analysis of extracts of various cell lines, using AMBRA1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunohistochemistry-Paraffin: AMBRA1 Antibody - BSA Free [NBP2-92849]

Immunohistochemistry-Paraffin: AMBRA1 Antibody - BSA Free [NBP2-92849]

Immunohistochemistry-Paraffin: AMBRA1 Antibody [NBP2-92849] - Paraffin-embedded rat lung using AMBRA1 .
Immunohistochemistry-Paraffin: AMBRA1 Antibody - BSA Free [NBP2-92849]

Immunohistochemistry-Paraffin: AMBRA1 Antibody - BSA Free [NBP2-92849]

Immunohistochemistry-Paraffin: AMBRA1 Antibody [NBP2-92849] - Paraffin-embedded mouse lung using AMBRA1 .

Applications for AMBRA1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AMBRA1

AMBRA1 regulates autophagy and development of the nervous system. It is a large, previously unknown protein bearing a WD40 domain at its amino terminus, regulates autophagy and has a crucial role in embryogenesis. AMBRA1 is a positive regulator of the Becn1-dependent programme of autophagy, as revealed by its overexpression and by RNA interference experiments invitro. Notably, AMBRA1 functional deficiency in mouse embryos leads to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic cell death.

Alternate Names

activating molecule in BECN1-regulated autophagy protein 1, autophagy/beclin-1 regulator 1, DCAF3, DDB1 and CUL4 associated factor 3, FLJ20294, KIAA1736activating molecule in beclin-1-regulated autophagy, MGC33725, WD repeat domain 94, WDR94

Gene Symbol

AMBRA1

Additional AMBRA1 Products

Product Documents for AMBRA1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AMBRA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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