Amiloride-sensitive cation channel 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46288

Novus Biologicals, part of Bio-Techne
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NBP1-46288-0.025ml
NBP1-46288
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Amiloride-sensitive cation channel 3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide from the amino acid region 50-100 of the extracellular domain of rat Amiloride-sensitive cation channel 3 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Amiloride-sensitive cation channel 3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:300-1:2000

Immunohistochemistry-Frozen

1:300-1:2000

Immunohistochemistry-Paraffin

1:300-1:2000

Western Blot

1:300-1:2000
Application Notes
IHC, WB, ICCIF reactivity reported in (PMID: 23095108). Amiloride-sensitive cation channel 3 antibody validated for IHC-FR from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NBP1-46288 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Amiloride-sensitive cation channel 3

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and ACCN1 has been observed as proton-gated channels sensitive to gadolinium. Alternative splicing of this gene generates three transcript variants encoding distinct isoforms. FUNCTION: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable). Interacts with STOM and DLG4. Interacts with LIN7B, MAGI1/BAIAP1, GOPC and ACCN1. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cytoplasm. Note=Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells. TISSUE SPECIFICITY: Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis. DEVELOPMENTAL STAGE: Expressed in fetal tissues, expression increases in lung and kidney adult tissues.

Long Name

Acid-sensing ion channel 3

Alternate Names

ACCN3, ASIC3, SLNAC1, TNAC1

Entrez Gene IDs

9311 (Human)

Gene Symbol

ASIC3

Additional Amiloride-sensitive cation channel 3 Products

Product Documents for Amiloride-sensitive cation channel 3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Amiloride-sensitive cation channel 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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