Aminoacylase/ACY1 Antibody (006)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89558
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Aminoacylase/ACY1 Antibody (006) [NBP2-89558]](https://resources.bio-techne.com/images/products/Aminoacylase-ACY1-Antibody-006-Immunocytochemistry-Immunofluorescence-NBP2-89558-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Aminoacylase/ACY1 Antibody (006) [NBP2-89558]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 006
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Aminoacylase/ACY1 (Accession#: NP_000657.1; Met1-Ser408).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Aminoacylase/ACY1 Antibody (006)
Immunocytochemistry/ Immunofluorescence: Aminoacylase/ACY1 Antibody (006) [NBP2-89558]
Immunocytochemistry/Immunofluorescence: Aminoacylase/ACY1 Antibody (006) [NBP2-89558] - Staining of Human ACY1 in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human ACY1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.![Flow Cytometry: Aminoacylase/ACY1 Antibody (006) [NBP2-89558]](https://resources.bio-techne.com/images/products/Aminoacylase-ACY1-Antibody-006-Flow-Cytometry-NBP2-89558-img0002.jpg "Flow Cytometry: Aminoacylase/ACY1 Antibody (006) [NBP2-89558]")
Flow Cytometry: Aminoacylase/ACY1 Antibody (006) [NBP2-89558]
Flow Cytometry: Aminoacylase/ACY1 Antibody (006) [NBP2-89558] - Analysis of Human ACY1 expression on HepG2 cells. The cells were treated, stained with purified anti-Human ACY1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.Applications for Aminoacylase/ACY1 Antibody (006)
Application
Recommended Usage
Flow Cytometry
1:25-1:100
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aminoacylase/ACY1
Alternate Names
ACY1
Gene Symbol
ACY1
Additional Aminoacylase/ACY1 Products
Product Documents for Aminoacylase/ACY1 Antibody (006)
Product Specific Notices for Aminoacylase/ACY1 Antibody (006)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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