Aminoacylase/ACY1 Antibody (15G2-R)

Immunohistochemistry: Aminoacylase/ACY1 Antibody (15G2-R) [NBP3-32000]

Immunohistochemistry: Aminoacylase/ACY1 Antibody (15G2-R) [NBP3-32000] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Aminoacylase/ACY1 antibody (NBP3-32000) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32000) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Aminoacylase/ACY1 Antibody (15G2-R) [NBP3-32000]

Western Blot: Aminoacylase/ACY1 Antibody (15G2-R) [NBP3-32000] - Western blot analysis of Aminoacylase/ACY1 on different lysates with Mouse anti-Aminoacylase/ACY1 antibody (NBP3-32000) at 1/1,000 dilution.

Lane 1: K-562 cell lysate (20 ug/Lane)
Lane 2: Human liver tissue lysate (40 ug/Lane)
Lane 3: Human kidney tissue lysate (40 ug/Lane)
Lane 4: Mouse liver tissue lysate (40 ug/Lane)
Lane 5: Mouse kidney tissue lysate (40 ug/Lane)
Lane 6: Rat liver tissue lysate (40 ug/Lane)
Lane 7: Rat kidney tissue lysate (40 ug/Lane)

Predicted band size: 46 kDa
Observed band size: 42 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32000) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.