Aminopeptidase P1/XPNPEP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51954

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Aminopeptidase P1/XPNPEP1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-LIRETQPISKQH corresponding to C-Terminus according to NP_001161076.1.

Epitope

C-LIRETQPISKQH

Specificity

This antibody is expected to recognize isoform 1 and 2 (NP_065116.2; NP_001161076.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Aminopeptidase P1/XPNPEP1 Antibody

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954]

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954]

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954] - (0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954]

Immunohistochemistry-Paraffin: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954]

Immunohistochemistry-Paraffin: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954] - (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954]

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954]

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-51954] - Analysis of Mouse (A) and Rat (B) Small Intestine lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Applications for Aminopeptidase P1/XPNPEP1 Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 80 kDa band observed in human heart, skeletal muscle and pancreas lysates and 75 kDa band observed in mouse and rat small intestines (calculated MW of 69.9 kDa band according to NP_065116.2).Primary incubation was 1 hour.
IHC: Paraffin embedded Human Pancreas.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aminopeptidase P1/XPNPEP1

X-prolyl aminopeptidase (EC 3.4.11.9) is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. Because of its specificity toward proline, it has been suggested that X-prolyl aminopeptidase is important in the maturation and degradation of peptide hormones, neuropeptides, and tachykinins, as well as in the digestion of otherwise resistant dietary protein fragments, thereby complementing the pancreatic peptidases. Deficiency of X-prolyl aminopeptidase results in excretion of large amounts of imino-oligopeptides in urine (Blau et al., 1988 [PubMed 3141711]).[supplied by OMIM]

Alternate Names

APP1, SAMP, XPNPEP1

Entrez Gene IDs

7511 (Human); 170750 (Mouse); 170751 (Rat)

Gene Symbol

XPNPEP1

UniProt

NP_065116.2 (Human)

Additional Aminopeptidase P1/XPNPEP1 Products

Product Documents for Aminopeptidase P1/XPNPEP1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Aminopeptidase P1/XPNPEP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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