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Aminopeptidase P1/XPNPEP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80614

Novus Biologicals, part of Bio-Techne
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NBP1-80614
NBP1-80614-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AIIGLETIMLFIDGDRIDAPSVKEHLLLDLGLEAEYRIQVHPYKSILSELKALCADLSPREKVWVSDKASYAVSETIPKDHRCC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aminopeptidase P1/XPNPEP1 Antibody

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80614]

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80614]

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80614] - Analysis using Anti-XPNPEP1 antibody NBP1-80614 (A) shows similar pattern to independent antibody NBP1-80613 (B).
Immunocytochemistry/ Immunofluorescence: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80614]

Immunocytochemistry/ Immunofluorescence: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80614]

Immunocytochemistry/Immunofluorescence: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80614] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80614]

Immunohistochemistry-Paraffin: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80614]

Immunohistochemistry-Paraffin: Aminopeptidase P1/XPNPEP1 Antibody [NBP1-80614] - Staining of human duodenum shows cytoplasmic positivity in glandular cells.

Applications for Aminopeptidase P1/XPNPEP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aminopeptidase P1/XPNPEP1

XPNPEP1 is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. As a result of its specificity toward proline, it has been suggested that XPNPEP1 is important in the maturation and degradation of peptide hormones, neuropeptides, and tachykinins, as well as in the digestion of otherwise resistant dietary protein fragments, thereby complementing the pancreatic peptidases. Deficiency of XPNPEP1 results in excretion of large amounts of imino-oligopeptides in urine.

Alternate Names

APP1, SAMP, XPNPEP1

Entrez Gene IDs

7511 (Human)

Gene Symbol

XPNPEP1

UniProt

Additional Aminopeptidase P1/XPNPEP1 Products

Product Documents for Aminopeptidase P1/XPNPEP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aminopeptidase P1/XPNPEP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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