Aminopeptidase P1/XPNPEP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98604
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Aminopeptidase P1/XPNPEP1 Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human Aminopeptidase P1/XPNPEP1 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Aminopeptidase P1/XPNPEP1 Antibody
Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP2-98604]
Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP2-98604] - Anti-Aminopeptidase P1/XPNPEP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 72 kDa (We are unsure as to the identity of these extra bands).Immunocytochemistry/ Immunofluorescence: Aminopeptidase P1/XPNPEP1 Antibody [NBP2-98604]
Immunocytochemistry/Immunofluorescence: Aminopeptidase P1/XPNPEP1 Antibody [NBP2-98604] - Immunofluorescence staining of Aminopeptidase P1/XPNPEP1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Aminopeptidase P1/XPNPEP1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.Applications for Aminopeptidase P1/XPNPEP1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aminopeptidase P1/XPNPEP1
Alternate Names
APP1, SAMP, XPNPEP1
Gene Symbol
XPNPEP1
Additional Aminopeptidase P1/XPNPEP1 Products
Product Documents for Aminopeptidase P1/XPNPEP1 Antibody
Product Specific Notices for Aminopeptidase P1/XPNPEP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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