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Amisyn Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90175

Novus Biologicals, part of Bio-Techne
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NBP1-90175
NBP1-90175-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Amisyn Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NKKPTQASITKVKQFEGSTSFVRRSQWMLEQLRQVNGIDPNGDSAEFDLLFENAFDQWVASTASEKCTFFQILHHTCQRYLTDRKPEFINCQSKIMGGNSILHSAADSVTSAVQKASQALNERGERLGRAEEKTEDLK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Amisyn Antibody

Western Blot: Amisyn Antibody [NBP1-90175]

Western Blot: Amisyn Antibody [NBP1-90175]

Western Blot: Amisyn Antibody [NBP1-90175] - Analysis in human cell lines Caco-2 and PC-3 using anti-STXBP6 antibody. Corresponding STXBP6 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunohistochemistry-Paraffin: Amisyn Antibody [NBP1-90175]

Immunohistochemistry-Paraffin: Amisyn Antibody [NBP1-90175]

Immunohistochemistry-Paraffin: Amisyn Antibody [NBP1-90175] - Staining of human lateral ventricle shows strong positivity in neuronal cells.
Western Blot: Amisyn Antibody [NBP1-90175]

Western Blot: Amisyn Antibody [NBP1-90175]

Western Blot: Amisyn Antibody [NBP1-90175] - Analysis in control (vector only transfected HEK293T lysate) and STXBP6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Applications for Amisyn Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Amisyn

Amisyn binds components of the SNARE complex (see MIM 603215) and may be involved in regulating SNARE complex formation (Scales et al., 2002).

Alternate Names

amisyn, FLJ39638, HSPC156, syntaxin binding protein 6 (amisyn), syntaxin-binding protein 6

Gene Symbol

STXBP6

Additional Amisyn Products

Product Documents for Amisyn Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Amisyn Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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