AMPK alpha 1/2 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05635

Novus Biologicals, part of Bio-Techne
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NBP3-05635-100ul
NBP3-05635-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AMPK alpha 1/2 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 5-280 of human AMPK alpha 1/2 (NP_006242.5). SSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMPK alpha 1/2 Antibody - Azide and BSA Free

Western Blot: AMPK alpha 1/2 AntibodyAzide and BSA Free [NBP3-05635]

Western Blot: AMPK alpha 1/2 AntibodyAzide and BSA Free [NBP3-05635]

Western Blot: AMPK alpha 1/2 Antibody [NBP3-05635] - Western blot analysis of extracts of various cell lines, using AMPK alpha 1/2 antibody (NBP3-05635) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: AMPK alpha 1/2 Antibody - Azide and BSA Free [NBP3-05635]

Immunocytochemistry/ Immunofluorescence: AMPK alpha 1/2 Antibody - Azide and BSA Free [NBP3-05635]

Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05635] - Immunofluorescence analysis of U2OS cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05635) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: AMPK alpha 1/2 Antibody - Azide and BSA Free [NBP3-05635]

Immunocytochemistry/ Immunofluorescence: AMPK alpha 1/2 Antibody - Azide and BSA Free [NBP3-05635]

Immunocytochemistry/Immunofluorescence: AMPK alpha 1/2 Antibody [NBP3-05635] - Immunofluorescence analysis of NIH/3T3 cells using AMPK alpha 1/2 Rabbit pAb (NBP3-05635) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Applications for AMPK alpha 1/2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AMPK alpha 1/2

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic a subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms (alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations capable of forming complexes. The catalytic a subunit of AMPK is activated allosterically by AMP and by phosphorylation via the AMPK kinase LKB1. Active AMPK downregulates anabolic pathways such as fatty acid, triglyceride, and cholesterol synthesis, and up-regulates catabolic pathways such as glycolysis and fatty acid oxidation. AMPK's role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Long Name

AMP-activated Protein Kinase alpha 1/2

Alternate Names

AMPK, AMPKa1, PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit

Gene Symbol

PRKAA1

Additional AMPK alpha 1/2 Products

Product Documents for AMPK alpha 1/2 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AMPK alpha 1/2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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