AMPK alpha 1/2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12281

Novus Biologicals, part of Bio-Techne
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NBP3-12281
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AMPK alpha 1/2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 480-530 on rat 5'-AMP-activated protein kinase Felinealytic subunit alpha-2 protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for AMPK alpha 1/2 Antibody

Western Blot: AMPK alpha 1/2 Antibody [NBP3-12281]

Western Blot: AMPK alpha 1/2 Antibody [NBP3-12281]

Western Blot: AMPK alpha 1/2 Antibody [NBP3-12281] - Mouse brain. 1:1000 antibody dilution in Dilobuffer. Apparent MW is 62 KDa.
Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-12281]

Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-12281]

Immunohistochemistry-Paraffin: AMPK alpha 1/2 Antibody [NBP3-12281] - Rat small intestine. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

Applications for AMPK alpha 1/2 Antibody

Application
Recommended Usage

ELISA

1:50000

Immunohistochemistry

1:50-1:250

Immunohistochemistry-Paraffin

1:50-1:250

Immunoprecipitation

1:200

Western Blot

1:2500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: AMPK alpha 1/2

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic a subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms (alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations capable of forming complexes. The catalytic a subunit of AMPK is activated allosterically by AMP and by phosphorylation via the AMPK kinase LKB1. Active AMPK downregulates anabolic pathways such as fatty acid, triglyceride, and cholesterol synthesis, and up-regulates catabolic pathways such as glycolysis and fatty acid oxidation. AMPK's role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Long Name

AMP-activated Protein Kinase alpha 1/2

Alternate Names

AMPK, AMPKa1, PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit

Gene Symbol

PRKAA1

Additional AMPK alpha 1/2 Products

Product Documents for AMPK alpha 1/2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AMPK alpha 1/2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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