AMPK alpha 1 [p Ser496] Antibody (S01-3B7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19825

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-19825-100ul
NBP3-19825-50ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S01-3B7

Concentration

0.3 mg/ml

View all AMPK alpha 1 Antibodies »

Product Summary for AMPK alpha 1 [p Ser496] Antibody (S01-3B7)

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser496 of human AMPK alpha 1 (Uniprot # Q13131)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AMPK alpha 1 [p Ser496] Antibody (S01-3B7)

Western Blot: AMPK alpha 1 [p Ser496] Antibody (S01-3B7) [NBP3-19825] - Western blot detection of AMPK alpha 1 (phospho-Ser496) in Ramos cell lysates using NBP3-19825 (1:1000 diluted). Predicted band size: 64kDa. Observed band size: 64kDa.
Immunocytochemistry/Immunofluorescence: AMPK alpha 1 [p Ser496] Antibody (S01-3B7) [NBP3-19825] - Immunocytochemistry of AMPK alpha 1 (phospho-Ser496) (green) in hela using NBP3-19825 at dilution 1/50, and DAPI (blue)

Applications for AMPK alpha 1 [p Ser496] Antibody (S01-3B7)

Application
Recommended Usage

Western Blot

1/10001/50
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMPK alpha 1

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1) AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are non-catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways

Long Name

AMP-activated Protein Kinase alpha 1

Alternate Names

PRKAA1

Gene Symbol

PRKAA1

Additional AMPK alpha 1 Products

Product Documents for AMPK alpha 1 [p Ser496] Antibody (S01-3B7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for AMPK alpha 1 [p Ser496] Antibody (S01-3B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.