AMPK alpha 1 [p Ser496] Antibody (SR2088)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21561

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2088

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AMPK alpha 1 Antibodies »

Product Summary for AMPK alpha 1 [p Ser496] Antibody (SR2088)

Immunogen

A synthesized peptide derived from human phospho-AMPK alpha 1 [Ser496] (Uniprot #: Q13131)

Modification

p Ser 496

Specificity

Detects endogenous levels of phospho-AMPK alpha 1 [Ser496]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AMPK alpha 1 [p Ser496] Antibody (SR2088)

Western Blot: AMPK alpha 1 [p Ser 496] Antibody (SR2088) [NBP3-21561] -

Western Blot: AMPK alpha 1 [p Ser 496] Antibody (SR2088) [NBP3-21561] - Western blot analysis using NBP3-21561 in (1) 293T cell lysate treated with LP; (2) 293T cell lysate.

Applications for AMPK alpha 1 [p Ser496] Antibody (SR2088)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMPK alpha 1

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1) AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are non-catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways

Long Name

AMP-activated Protein Kinase alpha 1

Alternate Names

PRKAA1

Gene Symbol

PRKAA1

Additional AMPK alpha 1 Products

Product Documents for AMPK alpha 1 [p Ser496] Antibody (SR2088)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AMPK alpha 1 [p Ser496] Antibody (SR2088)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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