AMPK alpha 2 [p Thr172] Antibody (4F4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26400
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4F4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for AMPK alpha 2 [p Thr172] Antibody (4F4)
Immunogen
A synthesized peptide derived from Human AMPK alpha 2 [p Thr172] [UniProt P54646]
Modification
p Thr 172
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AMPK alpha 2 [p Thr172] Antibody (4F4)
![AMPK alpha 2 [p Thr172] Antibody (4F4)](https://resources.bio-techne.com/images/products/nbp3-26400_rabbit-ampk-alpha-2-p-thr-172-mab-4f4-282202411363013.jpg "Western Blot: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] -")
Western Blot: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] -
Western Blot: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] - Positive Western Blot detected in 293 whole cell lysate A549 whole cell lysate (treated with Calyculin A or EGF).All lanes: AMPK alpha 2 [p Thr172] Antibody at 1.95ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 62 KDa
Observed band size: 62 KDa
![AMPK alpha 2 [p Thr172] Antibody (4F4)](https://resources.bio-techne.com/images/products/nbp3-26400_rabbit-ampk-alpha-2-p-thr-172-mab-4f4-28220241323610.jpg "Immunocytochemistry/Immunofluorescence: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] -")
Immunocytochemistry/Immunofluorescence: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] -
Immunocytochemistry/Immunofluorescence: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] - Staining of A549 cells with AMPK alpha 2 [p Thr172] Antibody (4F4) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).Applications for AMPK alpha 2 [p Thr172] Antibody (4F4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: AMPK alpha 2
Long Name
AMP-activated Protein Kinase alpha
Alternate Names
PRKAA2
Gene Symbol
PRKAA2
Additional AMPK alpha 2 Products
Product Documents for AMPK alpha 2 [p Thr172] Antibody (4F4)
Product Specific Notices for AMPK alpha 2 [p Thr172] Antibody (4F4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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