AMPK alpha 2 Antibody (5B4G1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61853

Novus Biologicals, part of Bio-Techne
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NBP2-61853
NBP2-61853-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5B4G1

Format

BSA Free

Concentration

1 mg/ml

View all AMPK alpha 2 Antibodies »

Product Summary for AMPK alpha 2 Antibody (5B4G1) - BSA Free

Immunogen

Purified recombinant fragment of human AMPK alpha 2 (AA: 453-552) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

62.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AMPK alpha 2 Antibody (5B4G1) - BSA Free

Western Blot: AMPK alpha 2 Antibody (5B4G1)BSA Free [NBP2-61853]

Western Blot: AMPK alpha 2 Antibody (5B4G1)BSA Free [NBP2-61853]

Western Blot: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis using PRKAA2 mAb against human PRKAA2 (AA: 453-552) recombinant protein. (Expected MW is 36.7 kDa)
Immunohistochemistry: AMPK alpha 2 Antibody (5B4G1) - BSA Free [NBP2-61853]

Immunohistochemistry: AMPK alpha 2 Antibody (5B4G1) - BSA Free [NBP2-61853]

Immunohistochemistry: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis of paraffin-embedded rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.
Western Blot: AMPK alpha 2 Antibody (5B4G1)BSA Free [NBP2-61853]

Western Blot: AMPK alpha 2 Antibody (5B4G1)BSA Free [NBP2-61853]

Western Blot: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis using PRKAA2 mAb against HEK293 (1) and PRKAA2 (AA: 453-552)-hIgGFc transfected HEK293 (2) cell lysate.
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Applications for AMPK alpha 2 Antibody (5B4G1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMPK alpha 2

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Long Name

AMP-activated Protein Kinase alpha

Alternate Names

PRKAA2

Gene Symbol

PRKAA2

Additional AMPK alpha 2 Products

Product Documents for AMPK alpha 2 Antibody (5B4G1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AMPK alpha 2 Antibody (5B4G1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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