AMPK beta 1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92961
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for AMPK beta 1 Antibody - BSA Free
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human AMPK beta 1 (NP_006244.2). MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for AMPK beta 1 Antibody - BSA Free
Western Blot: AMPK beta 1 AntibodyBSA Free [NBP2-92961]
Western Blot: AMPK beta 1 Antibody [NBP2-92961] - Analysis of extracts of various cell lines, using AMPK beta 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.Immunocytochemistry/ Immunofluorescence: AMPK beta 1 Antibody - BSA Free [NBP2-92961]
Immunocytochemistry/Immunofluorescence: AMPK beta 1 Antibody [NBP2-92961] - Analysis of NIH-3T3 cells using AMPK beta 1 . Blue: DAPI for nuclear staining.Knockout Validated: AMPK beta 1 Antibody - BSA Free [NBP2-92961]
Knockout Validated: AMPK beta 1 Antibody [NBP2-92961] - Analysis of extracts from normal (control) and AMPK beta 1 knockout (KO) HeLa cells, using AMPK beta 1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milkApplications for AMPK beta 1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:100
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AMPK beta 1
Long Name
AMP-activated Protein Kinase beta 1
Alternate Names
PRKAB1
Gene Symbol
PRKAB1
Additional AMPK beta 1 Products
Product Documents for AMPK beta 1 Antibody - BSA Free
Product Specific Notices for AMPK beta 1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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