AMPK gamma 1 Antibody (5E5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26370

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26370-100ul
NBP3-26370-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5E5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AMPK gamma 1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human AMPK gamma 1 [UniProt P54619]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMPK gamma 1 Antibody (5E5)

AMPK gamma 1 Antibody (5E5)

Immunohistochemistry: AMPK gamma 1 Antibody (5E5) [NBP3-26370] -

Immunohistochemistry: AMPK gamma 1 Antibody (5E5) [NBP3-26370] - Image of AMPK gamma 1 Antibody (5E5) diluted at 1:100 and staining in paraffin-embedded human Prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Applications for AMPK gamma 1 Antibody (5E5)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: AMPK gamma 1

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1), is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpah subunit is the catalytic subunit, while beta and gamma are non-catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3).

Long Name

AMP-activated Protein Kinase gamma 1

Alternate Names

AMPKG, AMPKG1, PRKAG1

Gene Symbol

PRKAG1

Additional AMPK gamma 1 Products

Product Documents for AMPK gamma 1 Antibody (5E5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AMPK gamma 1 Antibody (5E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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