AMPK gamma 1 Antibody (S08-7D5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14994

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14994-100ul
NBP3-14994-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-7D5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AMPK gamma 1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human AMPK gamma 1 (Uniprot#: P54619)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AMPK gamma 1 Antibody (S08-7D5)

Western Blot: AMPK gamma 1 Antibody (S08-7D5) [NBP3-14994]

Western Blot: AMPK gamma 1 Antibody (S08-7D5) [NBP3-14994]

Western Blot: AMPK gamma 1 Antibody (S08-7D5) [NBP3-14994] - Western blot detection of AMPK gamma 1 in CHO-K1, C6, Jurkat cell lysates using AMPK gamma 1 Rabbit mAb (1:500 diluted). Predicted band size: 38kDa. Observed band size: 38kDa.

Applications for AMPK gamma 1 Antibody (S08-7D5)

Application
Recommended Usage

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMPK gamma 1

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1), is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpah subunit is the catalytic subunit, while beta and gamma are non-catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3).

Long Name

AMP-activated Protein Kinase gamma 1

Alternate Names

AMPKG, AMPKG1, PRKAG1

Gene Symbol

PRKAG1

Additional AMPK gamma 1 Products

Product Documents for AMPK gamma 1 Antibody (S08-7D5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AMPK gamma 1 Antibody (S08-7D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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