AMPK gamma 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00005571-D01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AMPK gamma 1 Antibodies »

Product Specifications

Immunogen

PRKAG1 (NP_997626.1, 1 a.a. - 247 a.a.) full-length human protein. MLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKKP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for AMPK gamma 1 Antibody

Western Blot: AMPK gamma 1 Antibody [H00005571-D01P]

Western Blot: AMPK gamma 1 Antibody [H00005571-D01P]

Western Blot: AMPK gamma 1 Antibody [H00005571-D01P] - Analysis of PRKAG1 expression in A-431.
Immunocytochemistry/ Immunofluorescence: AMPK gamma 1 Antibody [H00005571-D01P]

Immunocytochemistry/ Immunofluorescence: AMPK gamma 1 Antibody [H00005571-D01P]

Immunocytochemistry/Immunofluorescence: AMPK gamma 1 Antibody [H00005571-D01P] - Analysis of antibody to PRKAG1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: AMPK gamma 1 Antibody [H00005571-D01P]

Western Blot: AMPK gamma 1 Antibody [H00005571-D01P]

Western Blot: AMPK gamma 1 Antibody [H00005571-D01P] - Analysis of PRKAG1 expression in transfected 293T cell line by PRKAG1 polyclonal antibody.Lane 1: PRKAG1 transfected lysate(28.30 KDa).Lane 2: Non-transfected lysate.

Applications for AMPK gamma 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for WB and Functional.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AMPK gamma 1

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1), is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpah subunit is the catalytic subunit, while beta and gamma are non-catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3).

Long Name

AMP-activated Protein Kinase gamma 1

Alternate Names

AMPKG, AMPKG1, PRKAG1

Gene Symbol

PRKAG1

Additional AMPK gamma 1 Products

Product Documents for AMPK gamma 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AMPK gamma 1 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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