Amylin Antibody (R10/99)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-713
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # R10/99
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A peptide CATQRLANFLV corresponding to residues 40-50 of human IAPP coupled to tuberculin
Localization
Cytoplasmic and Secreted
Specificity
The is of high affinity, and stains islet amyloid peptide in intra-cellular insulin granules, intra-cellular amyloid and extra-cellular islet amyloid.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Amylin Antibody (R10/99)
![Immunocytochemistry/ Immunofluorescence: Amylin Antibody (R10/99) [NB600-713]](https://resources.bio-techne.com/images/products/Amylin-Antibody-R10-99-Immunocytochemistry-Immunofluorescence-NB600-713-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Amylin Antibody (R10/99) [NB600-713]")
Immunocytochemistry/ Immunofluorescence: Amylin Antibody (R10/99) [NB600-713]
Immunocytochemistry/Immunofluorescence: Amylin Antibody (R10/99) [NB600-713] - Staining of paraffin embedded human pancreas using Guinea Pig anti-Pig Insulin antibody (A) and Mouse anti-Human Amylin antibody (B). Merged image showing co-localization of the two proteins (C). DAPI was used as nuclear counterstain (blue)![Immunohistochemistry-Paraffin: Amylin Antibody (R10/99) [NB600-713]](https://resources.bio-techne.com/images/products/Amylin-Antibody-R10-99-Immunohistochemistry-Paraffin-NB600-713-img0001.jpg "Immunohistochemistry-Paraffin: Amylin Antibody (R10/99) [NB600-713]")
Immunohistochemistry-Paraffin: Amylin Antibody (R10/99) [NB600-713]
Immunohistochemistry-Paraffin: Amylin Antibody (R10/99) [NB600-713] - Human pancreas with Mouse anti Human Amylin Peptide, showing labeling of Islet of Langerhans.Applications for Amylin Antibody (R10/99)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:100
Immunohistochemistry-Paraffin
1:100
Application Notes
IHC-P: Use at a dilution of 1/100. Antigen retrieval is not essential but may optimise staining.IHC-Fr: Use at a dilution of 1/100. This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
Tissue culture supernatant
Preservative
<0.1% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Amylin
Alternate Names
Amylin, DAPamylin, Diabetes-associated peptide, IAP, Insulinoma amyloid peptide, islet amyloid polypeptide, Islet amyloid polypeptide (diabetes-associated peptide; amylin)
Entrez Gene IDs
3375 (Human)
Gene Symbol
IAPP
UniProt
Additional Amylin Products
Product Documents for Amylin Antibody (R10/99)
Product Specific Notices for Amylin Antibody (R10/99)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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