Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ANAPC13 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the center of human APC13. The immunogen is located within the last 50 amino acids of APC13. Amino Acid Squence: PLNELPEPEQDNGGTTE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ANAPC13 Antibody - BSA Free

ANAPC13 Antibody - BSA Free

Western Blot: ANAPC13 Antibody - BSA Free [NBP1-76261] -

Western Blot: ANAPC13 Antibody - BSA Free [NBP1-76261] - Western blot analysis of ANAPC13 in Jurkat cell tissue lysate with ANAPC13 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Western Blot: ANAPC13 AntibodyBSA Free [NBP1-76261]

Western Blot: ANAPC13 AntibodyBSA Free [NBP1-76261]

Western Blot: ANAPC13 Antibody [NBP1-76261] - Western blot analysis with 25ng of recombinant ANAPPC13 protein with ANAPC13 antibody at 1 ug/ml.
ANAPC13 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ANAPC13 Antibody - BSA Free [NBP1-76261] -

Immunocytochemistry/ Immunofluorescence: ANAPC13 Antibody - BSA Free [NBP1-76261] - Immunofluorescence of ANAPC13 in Jurkat cells with ANAPC13 antibody at 20 ug/mL.

Applications for ANAPC13 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ANAPC13

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC13 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC13 promotes the stable association of APC3/Cdc27 and APC6/Cdc16 with the APC/C.

Alternate Names

anaphase promoting complex subunit 13, anaphase-promoting complex subunit 13, APC13SWM1, cyclosome subunit 13, Cylosome subunit 13, DKFZP566D193, homolog of yeast Swm1

Gene Symbol

ANAPC13

UniProt

NP_056206 (Human)

Additional ANAPC13 Products

Product Documents for ANAPC13 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ANAPC13 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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