ANAPC13 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91664

Novus Biologicals, part of Bio-Techne
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NBP1-91664
NBP1-91664-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ANAPC13 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MDSEVQRDGRILDLIDDAWREDKLPYEDVAIPLNELPEPEQDNGGTTESVKEQEMKWTDLALQYLHENVP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ANAPC13 Antibody

Western Blot: ANAPC13 Antibody [NBP1-91664]

Western Blot: ANAPC13 Antibody [NBP1-91664]

Western Blot: ANAPC13 Antibody [NBP1-91664] - Analysis in control (vector only transfected HEK293T lysate) and ANAPC13 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ANAPC13 Antibody [NBP1-91664]

Immunocytochemistry/ Immunofluorescence: ANAPC13 Antibody [NBP1-91664]

Immunocytochemistry/Immunofluorescence: ANAPC13 Antibody [NBP1-91664] - Staining of human cell line U-2 OS shows positivity in mitochondria.
Immunohistochemistry-Paraffin: ANAPC13 Antibody [NBP1-91664]

Immunohistochemistry-Paraffin: ANAPC13 Antibody [NBP1-91664]

Immunohistochemistry-Paraffin: ANAPC13 Antibody [NBP1-91664] - Staining of human kidney shows cytoplasmic positivity in renal tubules.

Applications for ANAPC13 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ANAPC13

ANAPC13 encodes a component of the anaphase promoting complex, a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. The encoded protein is evolutionarily conserved and is required for the integrity and ubiquitin ligase activity of the anaphase promoting complex. Pseudogenes and splice variants have been found for this gene; however, the biological validity of some of the splice variants has not been determined. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the functional protein.

Alternate Names

anaphase promoting complex subunit 13, anaphase-promoting complex subunit 13, APC13SWM1, cyclosome subunit 13, Cylosome subunit 13, DKFZP566D193, homolog of yeast Swm1

Entrez Gene IDs

25847 (Human)

Gene Symbol

ANAPC13

UniProt

Q9BS18 (Human)

Additional ANAPC13 Products

Product Documents for ANAPC13 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ANAPC13 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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