ANAPC2 Antibody (OTI1A6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71860

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1A6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ANAPC2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ANAPC2(NP_037498) produced in HEk293 cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

93.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ANAPC2 Antibody (OTI1A6) - Azide and BSA Free

Western Blot: ANAPC2 Antibody (OTI1A6)Azide and BSA Free [NBP2-71860]

Western Blot: ANAPC2 Antibody (OTI1A6)Azide and BSA Free [NBP2-71860]

Western Blot: ANAPC2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-71860] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANAPC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC2.
Immunocytochemistry/ Immunofluorescence: ANAPC2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-71860]

Immunocytochemistry/ Immunofluorescence: ANAPC2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-71860]

Immunocytochemistry/Immunofluorescence: ANAPC2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-71860] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC2.
Immunohistochemistry: ANAPC2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-71860]

Immunohistochemistry: ANAPC2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-71860]

Immunohistochemistry: ANAPC2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-71860] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ANAPC2 mouse monoclonal antibody.
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Applications for ANAPC2 Antibody (OTI1A6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ANAPC2

ANAPC2 is a large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation.

Alternate Names

anaphase promoting complex subunit 2, anaphase-promoting complex subunit 2, APC2KIAA1406Cyclosome subunit 2, RP11-350O14.5

Gene Symbol

ANAPC2

Additional ANAPC2 Products

Product Documents for ANAPC2 Antibody (OTI1A6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ANAPC2 Antibody (OTI1A6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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