Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Ancient ubiquitous protein 1 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 426 and 476 of human Ancient Ubiquitous Protein 1 using the numbering given in entry NP_853553.1 (GeneID 550).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ancient ubiquitous protein 1 Antibody

Western Blot: Ancient ubiquitous protein 1 Antibody [NBP1-49916]

Western Blot: Ancient ubiquitous protein 1 Antibody [NBP1-49916]

Western Blot: Ancient ubiquitous protein 1 Antibody [NBP1-49916] - Detection of Human AUP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-AUP1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. AUP1 was also immunoprecipitated by rabbit anti-AUP1 antibody BL10556, which recognizes an upstream epitope. For blotting immunoprecipitated AUP1 wase used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Immunohistochemistry: Ancient ubiquitous protein 1 Antibody [NBP1-49916]

Immunohistochemistry: Ancient ubiquitous protein 1 Antibody [NBP1-49916]

Immunohistochemistry: Ancient ubiquitous protein 1 Antibody [NBP1-49916] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AUP1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for Ancient ubiquitous protein 1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Ancient ubiquitous protein 1

This gene encodes a protein that contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain that may be involved in binding ubiquitin-conjugating enzymes. The protein encoded by this gene has been shown to bind to the conserved membrane-proximal sequence of the cytoplasmic tail of integrin alpha(IIb) subunits. These subunits play a crucial role in the integrin alpha(IIb)beta(3) inside-out signaling in platelets and megakaryocytes that leads to platelet aggregation and thrombus formation. This gene overlaps the gene for mitochondrial serine protease 25.

Alternate Names

ancient ubiquitous protein 1

Gene Symbol

AUP1

Additional Ancient ubiquitous protein 1 Products

Product Documents for Ancient ubiquitous protein 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ancient ubiquitous protein 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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