Ancient ubiquitous protein 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06403

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ancient ubiquitous protein 1 Antibodies »

Product Summary for Ancient ubiquitous protein 1 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human Ancient ubiquitous protein 1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Ancient ubiquitous protein 1 Antibody

Western Blot: Ancient ubiquitous protein 1 Antibody [NBP3-06403]

Western Blot: Ancient ubiquitous protein 1 Antibody [NBP3-06403]

Western Blot: Ancient ubiquitous protein 1 Antibody [NBP3-06403] - AUP1 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate. 4 uL anti-AUP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-AUP1 rabbit polyclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :43 kDa.
Immunohistochemistry-Paraffin: Ancient ubiquitous protein 1 Antibody [NBP3-06403]

Immunohistochemistry-Paraffin: Ancient ubiquitous protein 1 Antibody [NBP3-06403]

Immunohistochemistry-Paraffin: Ancient ubiquitous protein 1 Antibody [NBP3-06403] - Immunochemical staining of human AUP1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Western Blot: Ancient ubiquitous protein 1 Antibody [NBP3-06403]

Western Blot: Ancient ubiquitous protein 1 Antibody [NBP3-06403]

Western Blot: Ancient ubiquitous protein 1 Antibody [NBP3-06403] - Anti-AUP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: RPMI-8226 Whole Cell Lysate Lane B: NIH/3T3 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:53 kDa.
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Applications for Ancient ubiquitous protein 1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ancient ubiquitous protein 1

Ancient ubiquitous protein 1 encodes a protein that contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain that may be involved in binding ubiquitin-conjugating enzymes. The protein encoded by this gene has been shown to bind to the conserved membrane-proximal sequence of the cytoplasmic tail of integrin alpha(IIb) subunits. These subunits play a crucial role in the integrin alpha(IIb)beta(3) inside-out signaling in platelets and megakaryocytes that leads to platelet aggregation and thrombus formation. This gene overlaps the gene for mitochondrial serine protease 25.

Alternate Names

ancient ubiquitous protein 1

Gene Symbol

AUP1

Additional Ancient ubiquitous protein 1 Products

Product Documents for Ancient ubiquitous protein 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ancient ubiquitous protein 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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