Angiopoietin-2 Antibody (117)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89600

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-89600-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 117

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Angiopoietin-2 Antibodies »

Product Summary for Angiopoietin-2 Antibody (117)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Angiopoietin-2 (Accession#: NP_001138.1; Met1-Phe496).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Angiopoietin-2 Antibody (117)

Western Blot: Angiopoietin-2 Antibody (117) [NBP2-89600]

Western Blot: Angiopoietin-2 Antibody (117) [NBP2-89600]

Western Blot: Angiopoietin-2 Antibody (117) [NBP2-89600] - Lane A: HepG2 Whole Cell Lysate Lane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:57 kDa Observed band size:70 kDa
Immunocytochemistry/ Immunofluorescence: Angiopoietin-2 Antibody (117) [NBP2-89600]

Immunocytochemistry/ Immunofluorescence: Angiopoietin-2 Antibody (117) [NBP2-89600]

Immunocytochemistry/Immunofluorescence: Angiopoietin-2 Antibody (117) [NBP2-89600] - Staining of Human Ang2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Ang2 monoclonal antibody (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 549-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).
Immunoprecipitation: Angiopoietin-2 Antibody (117) [NBP2-89600]

Immunoprecipitation: Angiopoietin-2 Antibody (117) [NBP2-89600]

Immunoprecipitation: Angiopoietin-2 Antibody (117) [NBP2-89600] - Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate 0.5 uL anti-Ang2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Ang2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 57 kDaObserved band size: 57 kDa

Applications for Angiopoietin-2 Antibody (117)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Angiopoietin-2

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Alternate Names

ANGPT2

Gene Symbol

ANGPT2

Additional Angiopoietin-2 Products

Product Documents for Angiopoietin-2 Antibody (117)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Angiopoietin-2 Antibody (117)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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