Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71414

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1B2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Angiopoietin-like Protein 1/ANGPTL1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 24-491 of human ANGPTL1(NP_004664) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

54.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2)Azide and BSA Free [NBP2-71414]

Western Blot: Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2)Azide and BSA Free [NBP2-71414]

Western Blot: Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free [NBP2-71414] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANGPTL1 .
Immunohistochemistry: Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free [NBP2-71414]

Immunohistochemistry: Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free [NBP2-71414]

Immunohistochemistry: Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free [NBP2-71414] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free [NBP2-71414]

Immunohistochemistry: Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free [NBP2-71414]

Immunohistochemistry: Angiopoietin-like Protein 1/ANGPTL1 Antibody (OTI1B2) - Azide and BSA Free [NBP2-71414] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Angiopoietin-like Protein 1/ANGPTL1

Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. The protein encoded by this gene is another member of the angiopoietin family that is widely expressed in adult tissues with mRNA levels highest in highly vascularized tissues. This protein was found to be a secretory protein that does not act as an endothelial cell mitogen in vitro.

Alternate Names

Angiopoietin like Protein 1, ANGPTL1, AngY1, ARP1

Gene Symbol

ANGPTL1

Additional Angiopoietin-like Protein 1/ANGPTL1 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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