Angiopoietin-like Protein 3/ANGPTL3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15390

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Angiopoietin-like Protein 3/ANGPTL3 Antibodies »

Product Summary for Angiopoietin-like Protein 3/ANGPTL3 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Angiopoietin-like Protein 3/ANGPTL3. The exact sequence is proprietary.

Reactivity Notes

Mouse (80%), Rat (80%), Pig (85%), Bovine (86%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Angiopoietin-like Protein 3/ANGPTL3 Antibody

Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390]

Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390]

Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390] - Human hepatoma, using ANGPTL3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Angiopoietin-like Protein 3/ANGPTL3 Antibody

Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390] -

Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390] - HepG2 whole cell extract and conditioned medium (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Angiopoietin-like Protein 3/ANGPTL3 antibody (NBP2-15390) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Angiopoietin-like Protein 3/ANGPTL3 Antibody

Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390] -

Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody [NBP2-15390] - Angiopoietin-like Protein 3/ANGPTL3 antibody detects Angiopoietin-like Protein 3/ANGPTL3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
Angiopoietin-like Protein 3/ANGPTL3 stained by Angiopoietin-like Protein 3/ANGPTL3 antibody (NBP2-15390) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Angiopoietin-like Protein 3/ANGPTL3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Angiopoietin-like Protein 3/ANGPTL3

The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain. The FBN-like domain in angiopoietin-like 3 protein was shown to bind alpha-5/beta-3 integrins, and this binding induced endothelial cell adhesion and migration. This protein may also play a role in the regulation of angiogenesis. [provided by RefSeq]

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Gene Symbol

ANGPTL3

UniProt

Q9Y5C1 (Human)

Additional Angiopoietin-like Protein 3/ANGPTL3 Products

Product Documents for Angiopoietin-like Protein 3/ANGPTL3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Angiopoietin-like Protein 3/ANGPTL3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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