Anillin Antibody (CL0301)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52907

Novus Biologicals, part of Bio-Techne
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NBP2-52907
NBP2-52907-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0301

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Anillin Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: IVKSTLSQTVPSKGELSREICLQSQSKDKSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSAEKGGNSKSKQLETKQETH

Epitope

PSKGELSREICLQSQ

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Anillin Antibody (CL0301)

Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907]

Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907]

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907] - Staining of U-251 cells using the Anti-ATAD2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining in human testis and skeletal muscle tissues. Corresponding ANLN RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907]

Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907]

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907] - Staining of A-431 cells using the Anti-ATAD2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Applications for Anillin Antibody (CL0301)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Anillin

Anillin is a 124 kDa scaffolding protein that plays an important role in cell division. Anillin interacts with septins and mediates septin filament assembly and stabilization in the construction of the contractile ring responsible for cleavage furrow formation and cytokinesis.

Alternate Names

actin-binding protein anillin, ANILLIN, anillin (Drosophila Scraps homolog), actin binding protein, anillin, actin binding protein, anillin, actin binding protein (scraps homolog, Drosophila), DKFZp779A055, Drosophila Scraps homolog, scra, Scraps

Gene Symbol

ANLN

Additional Anillin Products

Product Documents for Anillin Antibody (CL0301)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Anillin Antibody (CL0301)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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