ANKK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28459

Novus Biologicals, part of Bio-Techne
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NBP1-28459
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ANKK1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SRQADPNLHEAEGKT corresponding to internal region according to NP_848605.1.

Epitope

C terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ANKK1 Antibody

Western Blot: ANKK1 Antibody [NBP1-28459]

Western Blot: ANKK1 Antibody [NBP1-28459]

Western Blot: ANKK1 Antibody [NBP1-28459] - Analysis of ANKK1 in Human Substantia Nigra lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ANKK1 Antibody [NBP1-28459]

Immunohistochemistry-Paraffin: ANKK1 Antibody [NBP1-28459]

Immunohistochemistry-Paraffin: ANKK1 Antibody [NBP1-28459] - (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ANKK1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5-10 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1-0.3 ug/ml
Application Notes
Western blot: Approx. 85kDa band observed in Human Brain (Substantia nigra and Amygdala) lysates (calculated MW of 84.6 kDa according to NP_848605.1). Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: Paraffin embedded Human Prostate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ANKK1

The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and protein kinase superfamily involved in signal transduction pathways. This gene is closely linked to DRD2 gene (GeneID:1813) on chr 11, and a well studied restriction fragment length polymorphism (RFLP) designated TaqIA, was originally associated with the DRD2 gene, however, later was determined to be located in exon 8 of ANKK1 gene (PMIDs: 18621654, 15146457), where it causes a nonconservative amino acid substitution. It is not clear if this gene plays any role in neuropsychiatric disorders previously associated with Taq1A RFLP.

Alternate Names

ankyrin repeat and kinase domain containing 1, ankyrin repeat and protein kinase domain-containing protein 1, EC 2.7.11, EC 2.7.11.1, PKK2, Protein kinase PKK2, SGK288, Sugen kinase 288, X-kinasesgK288

Entrez Gene IDs

255239 (Human); 93898 (Mouse); 306351 (Rat)

Gene Symbol

ANKK1

UniProt

NP_848605.1 (Human)

Additional ANKK1 Products

Product Documents for ANKK1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ANKK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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