ANKLE2 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92367
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ANKLE2 Antibody - Azide and BSA Free
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 350-450 of human ANKLE2 (NP_055929.1). CRYNVMHVAAKENQASICQLTLDVLENPDFMRLMYPDDDEAMLQKRIRYVVDLYLNTPDKMGYDTPLHFACKFGNADVVNVLSSHHLIVKNSRNKYDKTPE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ANKLE2 Antibody - Azide and BSA Free
Western Blot: ANKLE2 AntibodyAzide and BSA Free [NBP2-92367]
Western Blot: ANKLE2 Antibody [NBP2-92367] - Analysis of extracts of various cell lines, using ANKLE2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.Immunohistochemistry-Paraffin: ANKLE2 Antibody - Azide and BSA Free [NBP2-92367]
Immunohistochemistry-Paraffin: ANKLE2 Antibody [NBP2-92367] - Immunohistochemistry of paraffin-embedded Human lung cancer using ANKLE2 Rabbit pAb (NBP2-92367) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Immunohistochemistry-Paraffin: ANKLE2 Antibody - Azide and BSA Free [NBP2-92367]
Immunohistochemistry-Paraffin: ANKLE2 Antibody [NBP2-92367] - Immunohistochemistry of paraffin-embedded Mouse kidney using ANKLE2 Rabbit pAb (NBP2-92367) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for ANKLE2 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ANKLE2
Alternate Names
ankyrin repeat and LEM domain containing 2, ankyrin repeat and LEM domain-containing protein 2, FLJ22280, FLJ36132, KIAA0692, LEM domain containing 7, LEMD7
Gene Symbol
ANKLE2
Additional ANKLE2 Products
Product Documents for ANKLE2 Antibody - Azide and BSA Free
Product Specific Notices for ANKLE2 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov