Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ANKRD15 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1302 and 1352 of human KN motif and ankyrin repeat domains 1 using the numbering given in entry NP_055973.2 (GeneID 23189).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ANKRD15 Antibody

Western Blot: ANKRD15 Antibody [NBP1-03345]

Western Blot: ANKRD15 Antibody [NBP1-03345]

Western Blot: ANKRD15 Antibody [NBP1-03345] - Detection of Human KANK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KANK1 antibody NBP1-03345 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: ANKRD15 Antibody [NBP1-03345]

Western Blot: ANKRD15 Antibody [NBP1-03345]

Western Blot: ANKRD15 Antibody [NBP1-03345] - Detection of Human KANK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- KANK1 antibody NBP1-03345 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. KANK1 was also immunoprecipitated by another rabbit anti-KANK1 antibody which recognizes an upstream epitope.
ANKRD15 Antibody [NBP1-03345] - Detection of human KANK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KANK1 antibody NBP1-03345 (lot NBP1-03345-2) used for IP at 6 ug per reaction. KANK1 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-03345-1) and another rabbit anti-KANK1 antibody. For blotting immunoprecipitated KANK1, NBP1-03345 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ANKRD15 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ANKRD15

The ANKRD15 gene encodes a protein containing four ankyrin repeat domains in its C-terminus. The suggested role for this protein is in tumorigenesis of renal cell carcinoma. Two alternatively spliced transcript variants encoding different isoforms have been iden

Alternate Names

ANKRD15ankyrin repeat domain 15, Ankyrin repeat domain-containing protein 15, KANKDKFZp451G231, KIAA0172MGC43128, Kidney ankyrin repeat-containing protein, KN motif and ankyrin repeat domain-containing protein 1, KN motif and ankyrin repeat domains 1

Entrez Gene IDs

23189 (Human)

Gene Symbol

KANK1

UniProt

Q14678 (Human)

Additional ANKRD15 Products

Product Documents for ANKRD15 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ANKRD15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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