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ANKRD2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91670

Novus Biologicals, part of Bio-Techne
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NBP1-91670
NBP1-91670-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IIKLLLLHGADMMTKNLAGKTPTDLVQLWQADTRHALEHPEPGAEHNGLEGPNDSGRETPQPVPAQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ANKRD2 Antibody

Immunohistochemistry-Paraffin: ANKRD2 Antibody [NBP1-91670]

Immunohistochemistry-Paraffin: ANKRD2 Antibody [NBP1-91670]

Immunohistochemistry-Paraffin: ANKRD2 Antibody [NBP1-91670] - Staining in human skeletal muscle and prostate tissues using anti-ANKRD2 antibody. Corresponding ANKRD2 RNA-seq data are presented for the same tissues.
Western Blot: ANKRD2 Antibody [NBP1-91670]

Western Blot: ANKRD2 Antibody [NBP1-91670]

Western Blot: ANKRD2 Antibody [NBP1-91670] - Analysis in control (vector only transfected HEK293T lysate) and ANKRD2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ANKRD2 Antibody [NBP1-91670]

Immunocytochemistry/ Immunofluorescence: ANKRD2 Antibody [NBP1-91670]

Immunocytochemistry/Immunofluorescence: ANKRD2 Antibody [NBP1-91670] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.

Applications for ANKRD2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ANKRD2

ANKRD2 belongs to the conserved muscle ankyrin repeat protein (MARP) family. Expression of MARPs is induced in response to physiologic stress, injury, and hypertrophy (Miller et al., 2003 [PubMed 14583192]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Alternate Names

ankyrin repeat domain 2 (stretch responsive muscle), ankyrin repeat domain-containing protein 2, ankyrin-repeat protein, ARPPSkeletal muscle ankyrin repeat protein, hArpp, MGC104314

Gene Symbol

ANKRD2

Additional ANKRD2 Products

Product Documents for ANKRD2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ANKRD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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