ANKRD27 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50030
![Western Blot: ANKRD27 Antibody [NBP1-50030]](https://resources.bio-techne.com/images/products/ANKRD27-Antibody-Western-Blot-NBP1-50030-img0003.jpg "Western Blot: ANKRD27 Antibody [NBP1-50030]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Predicted:
Primate (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1000 and 1050 of human Vps9 Domain and Ankyrin-Repeat-Containing Protein using the numbering given in entry NP_115515.2 (GeneID 84079).
Reactivity Notes
Orangutan (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ANKRD27 Antibody
Western Blot: ANKRD27 Antibody [NBP1-50030]
Western Blot: ANKRD27 Antibody [NBP1-50030] - Detection of Human Varp by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Varp antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Varp was also immunoprecipitated by rabbit anti-Varp antibody which recognizes an upstream epitope. For blotting immunoprecipitated Varp, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for ANKRD27 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ANKRD27
Alternate Names
ankyrin repeat domain 27 (VPS9 domain), ankyrin repeat domain-containing protein 27, DKFZp434L0718, FLJ00040, VARP, Vps9 domain and ankyrin-repeat-containing protein, VPS9 domain-containing protein, VPS9-ankyrin-repeat protein
Gene Symbol
ANKRD27
Additional ANKRD27 Products
Product Documents for ANKRD27 Antibody
Product Specific Notices for ANKRD27 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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