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ANKRD40 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-93516

Novus Biologicals, part of Bio-Techne
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NBP1-93516

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IMGVEEEDDDDDDDDNLPQLKKESELPFVPNYLANPAFPFIYTPTAEDSAQMQNGGPSTPPASPPADGSPPLLP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ANKRD40 Antibody

Western Blot: ANKRD40 Antibody [NBP1-93516]

Western Blot: ANKRD40 Antibody [NBP1-93516]

Western Blot: ANKRD40 Antibody [NBP1-93516] - Analysis in control (vector only transfected HEK293T lysate) and ANKRD40 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ANKRD40 Antibody [NBP1-93516]

Immunocytochemistry/ Immunofluorescence: ANKRD40 Antibody [NBP1-93516]

Immunocytochemistry/Immunofluorescence: ANKRD40 Antibody [NBP1-93516] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & the Golgi apparatus.
Immunohistochemistry-Paraffin: ANKRD40 Antibody [NBP1-93516]

Immunohistochemistry-Paraffin: ANKRD40 Antibody [NBP1-93516]

Immunohistochemistry-Paraffin: ANKRD40 Antibody [NBP1-93516] - Staining of human rectum shows strong nuclear positivity in glandular cells.

Applications for ANKRD40 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ANKRD40

Alternate Names

ankyrin repeat domain 40, MGC15396

Gene Symbol

ANKRD40

Additional ANKRD40 Products

Product Documents for ANKRD40 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ANKRD40 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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