ANKRD45 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85421
Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KEDKNTILSACRAKNEWLETHTEASINELFEQRQQLEDIVTPIFTKMTTPCQVKSAKSVTSHDQKRSQDDTSN
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ANKRD45 Antibody
Western Blot: ANKRD45 Antibody [NBP1-85421]
Western Blot: ANKRD45 Antibody [NBP1-85421] - Analysis in control (vector only transfected HEK293T lysate) and ANKRD45 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: ANKRD45 Antibody [NBP1-85421]
Immunohistochemistry-Paraffin: ANKRD45 Antibody [NBP1-85421] - Staining of human liver.Western Blot: ANKRD45 Antibody [NBP1-85421]
Western Blot: ANKRD45 Antibody [NBP1-85421] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Applications for ANKRD45 Antibody
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ANKRD45
Alternate Names
ankyrin repeat domain 45, ankyrin repeat domain-containing protein 45, cancer/testis antigen 117, CT117, FLJ45235, MGC161631, MGC161633, RP3-436N22.4
Entrez Gene IDs
339416 (Human)
Gene Symbol
ANKRD45
UniProt
Additional ANKRD45 Products
Product Documents for ANKRD45 Antibody
Product Specific Notices for ANKRD45 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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